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Generation of Neurospheres from Mixed Primary Hippocampal and Cortical Neurons Isolated from E14-E16 Sprague Dawley Rat Embryo
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Chapters
- 00:03Title
- 01:31Preparation of Poly-D-Lysine Plates for Neuron and Neurosphere Culture
- 02:38Removal and Decapitation of the Fetus
- 03:28Removal of Brain and Dissection of Cortex along with Hippocampus
- 04:32Dissociation of Cortical and Hippocampal Tissues into Single Neurons
- 07:54Results: Culture and Characterization of the Obtained Primary Neurons and Neurospheres
- 11:12Conclusion
Presented here is a protocol for the spontaneous generation of neurospheres enriched in neural progenitor cells from high density plated neurons. During the same experiment, when neurons are plated at a lower density, the protocol also results in prolonged primary rat neuron cultures.
