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Primary Culture of Neurons Isolated from Embryonic Mouse Cerebellum
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Neuroscience
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JoVE Journal Neuroscience
Primary Culture of Neurons Isolated from Embryonic Mouse Cerebellum

Primary Culture of Neurons Isolated from Embryonic Mouse Cerebellum

DOI:
10.3791/60168-v

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08:09 min

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October 26, 2019

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, , , , , , ,
1Department of Human Anatomy and Cell Science, Max Rady College of Medicine, Rady Faculty of Health Sciences,University of Manitoba, 2The Children’s Hospital Research Institute of Manitoba (CHRIM), Max Rady College of Medicine, Rady Faculty of Health Sciences,University of Manitoba, 3Department of Oral Biology,University of Illinois at Chicago, 4Department of Pathology, Max Rady College of Medicine, Rady Faculty of Health Sciences,University of Manitoba

Chapters

  • 00:00Title
  • 00:29Protocol
  • 06:32Results
  • 07:41Conclusion

Summary

Automatic Translation

Automatic Translation

Conducting in vitro experiments to reflect in vivo conditions as adequately as possible is not an easy task. The use of primary cell cultures is an important step toward understanding cell biology in a whole organism. The provided protocol outlines how to successfully grow and culture embryonic mouse cerebellar neurons.

Tags

Keywords: Primary Neuronal Cell CultureEmbryonic Mouse CerebellumIn VitroDissociated NeuronsCellular FeaturesMechanismsFunctionsMorphologyPoly-L-ornithineTrypsinDMEM/F12HBSSPBSDissection Medium

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