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Biology

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Simple Detection of Primary Cilia by Immunofluorescence
 

Simple Detection of Primary Cilia by Immunofluorescence

Article DOI: 10.3791/61155-v
May 15th, 2020

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Summary May 15th, 2020

Primary cilia are extracellular structures associated with the centriole. Primary cilia detection by immunofluorescent staining is a relatively simple procedure that results in extremely high-quality images. In this protocol, fibroblasts expressing primary cilia were fixed, immunostained, and imaged in a fluorescent or confocal microscope.

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