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JoVE Journal
Genetics

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A Universal Protocol for Large-scale gRNA Library Production from any DNA Source
 

A Universal Protocol for Large-scale gRNA Library Production from any DNA Source

Article doi: 10.3791/56264
December 6th, 2017

Summary December 6th, 2017

Methods for generating large-scale gRNA libraries should be simple, efficient and cost-effective. We describe a protocol for the production of gRNA libraries based on enzymatic digestion of target DNA. This method, CORALINA (comprehensive gRNA library generation through controlled nuclease activity) presents an alternative to costly custom oligonucleotide synthesis.

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