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Genetics
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Ultralow Input Genome Sequencing Library Preparation from a Single Tardigrade Specimen
 

Ultralow Input Genome Sequencing Library Preparation from a Single Tardigrade Specimen

Article DOI: 10.3791/57615 10:28 min
July 15th, 2018

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Summary July 15th, 2018

Contamination during the genomic sequencing of microscopic organisms remains a large problem. Here, we show a method to sequence the genome of a tardigrade from a single specimen with as little as 50 pg of genomic DNA without whole genome amplification to minimize the risk of contamination.

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