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A Customizable Protocol for String Assembly gRNA Cloning (STAgR)

A Customizable Protocol for String Assembly gRNA Cloning (STAgR)

Article DOI: 10.3791/58556-v 10:00 min December 26th, 2018
December 26th, 2018



Here, we present string assembly gRNA cloning (STAgR), a method to easily multiplex gRNA vectors for CRISPR/Cas9 approaches. STAgR makes gRNA multiplexing simple, efficient and customizable.



Customizable Protocol String Assembly GRNA Cloning STAgR Bacterial CRISPR-Cas9 System Guide RNAs Multiplex GRNA Expression Vectors N20 GRNA Sequences Forward Amplification Primers Overhangs Sense GRNA Sequences Reverse Primer Sequences Gibson Assembly High-fidelity Buffer DNTPs Overhang Primers DNA Template String Vector DMSO Water HF Polymerase Thermocycler
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