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JoVE Journal
Genetics

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A Customizable Protocol for String Assembly gRNA Cloning (STAgR)
 

A Customizable Protocol for String Assembly gRNA Cloning (STAgR)

Article DOI: 10.3791/58556-v
December 26th, 2018

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Summary December 26th, 2018

Here, we present string assembly gRNA cloning (STAgR), a method to easily multiplex gRNA vectors for CRISPR/Cas9 approaches. STAgR makes gRNA multiplexing simple, efficient and customizable.

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