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Dendrimer-based Uneven Nanopatterns to Locally Control Surface Adhesiveness: A Method to Direct Chondrogenic Differentiation
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Dendrimer-based Uneven Nanopatterns to Locally Control Surface Adhesiveness: A Method to Direct Chondrogenic Differentiation
DOI:

14:46 min

January 20, 2018

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Chapters

  • 00:00Title
  • 01:12Production of Nanopatterned Growth Substrates
  • 05:59Local RGD Surface Density Characterization with AFM
  • 07:49Cell Culture and Induction of Chondrogenesis
  • 08:49Cell Fixation and Immunostaining
  • 10:18Cell Imaging and Data Analysis
  • 12:28Representative Results
  • 13:29Conclusion

Summary

Automatic Translation

A method to obtain dendrimer-based uneven nanopatterns that permit the nanoscale control of local arginine-glycine-aspartic acid (RGD) surface density is described and applied for the study of cell adhesion and chondrogenic differentiation.

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