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Preparation of Thermoresponsive Nanostructured Surfaces for Tissue Engineering
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Chapters
- 00:05Title
- 01:15Synthesis of Polystyrene-block-poly(N-isopropylacrylamide) by Two-step Reversible Addition-fragmentation Chain Transfer (RAFT) Radical Polymerization
- 02:42Preparation of Silanized Hydrophobic Modified Glass Substrates
- 03:49Preparation of Langmuir Films and Film-transferred Surface
- 06:59Culturing Cells and Optimizing Cell Adhesion and Detachment on the Langmuir Film Transferred Surface
- 09:32Results: Fabrication of Langmuir Film-transferred Surface and Evaluation of Cell Adhesion, Detachment, and Cell Sheet Recovery
- 11:20Conclusion
Nanoscaled sea-island surfaces composed of thermoresponsive block copolymers were fabricated by the Langmuir-Schaefer method for controlling spontaneous cell adhesion and detachment. Both the preparation of the surface and the adhesion and detachment of cells on the surface were visualized.
