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Using a Microfluidics Device for Mechanical Stimulation and High Resolution Imaging of C. elegans
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JoVE Journal Neuroscience
Using a Microfluidics Device for Mechanical Stimulation and High Resolution Imaging of C. elegans

Using a Microfluidics Device for Mechanical Stimulation and High Resolution Imaging of C. elegans

DOI:
10.3791/56530-v

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10:39 min

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February 19, 2018

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, , , , ,
1Department of Molecular and Cellular Physiology,Stanford University, 2Department of Mechanical Engineering,Stanford University, 3Department of Bioengineering,Stanford University, 4Group of Neurophotonics and Mechanical Systems Biology,The Institute of Photonic Sciences (ICFO)

Chapters

  • 00:05Title
  • 00:52Preparation of the Microscope
  • 02:02Preparing the Microfluidic Chip
  • 03:45Loading the Test Subject
  • 06:02Stimulation and Removal of the Test Subject
  • 08:13Results: Touch Receptor Neuron (TRN) Stimuli
  • 09:42Conclusion

Summary

Automatic Translation

Automatic Translation

New tools for mechanobiology research are needed to understand how mechanical stress activates biochemical pathways and elicits biological responses. Here, we showcase a new method for selective mechanical stimulation of immobilized animals with a microfluidic trap allowing high-resolution imaging of cellular responses.

Tags

Keywords: MicrofluidicsMechanical StimulationHigh-resolution ImagingC. ElegansMechanobiologySensory BiologyPressure ActuatorsGCaMPRFPFluorescence MicroscopyM9 BufferPeristaltic PumpPDMS

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