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Using an Adapted Microfluidic Olfactory Chip for the Imaging of Neuronal Activity in Response to Pheromones in Male C. Elegans Head Neurons
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Neuroscience
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JoVE Journal Neuroscience
Using an Adapted Microfluidic Olfactory Chip for the Imaging of Neuronal Activity in Response to Pheromones in Male C. Elegans Head Neurons

Using an Adapted Microfluidic Olfactory Chip for the Imaging of Neuronal Activity in Response to Pheromones in Male C. Elegans Head Neurons

DOI:
10.3791/56026-v

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07:31 min

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September 07, 2017

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, , ,
1Department of Biology and Biotechnology,Worcester Polytechnic Institute, 2Department of Biomedical Engineering,Worcester Polytechnic Institute

Chapters

  • 00:05Title
  • 00:41Microfluidic Device Set Up
  • 02:54Loading C. elegans into the Microfluidic Device
  • 04:44Olfactory Stimulation of C. elegans Using a Microfluidic Device
  • 05:43Results: Neural Response to Chemical Stimuli
  • 06:45Conclusion

Summary

Automatic Translation

Automatic Translation

The use of an adapted "olfactory chip" for the efficient calcium imaging of C. elegans males is described here. Studies of male exposure to glycerol and a pheromone are also shown.

Tags

Keywords: MicrofluidicOlfactoryC. ElegansPheromonesCalcium TransienceNeuronal ActivitySex-specificNeural CircuitBiogenic PheromonesFluorescinS BasalMicro-fluidic DeviceFlow ControlVacuumGFP FilterNGM Agar Plate

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