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Microfluidic-based Electrotaxis for On-demand Quantitative Analysis of Caenorhabditis elegans‘ Locomotion
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Bioengineering
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JoVE Journal Bioengineering
Microfluidic-based Electrotaxis for On-demand Quantitative Analysis of Caenorhabditis elegans‘ Locomotion

Microfluidic-based Electrotaxis for On-demand Quantitative Analysis of Caenorhabditis elegans‘ Locomotion

DOI:
10.3791/50226-v

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10:23 min

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May 02, 2013

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, , , ,
1Department of Biology,McMaster University , 2Department of Mechanical Engineering,McMaster University

Chapters

  • 00:05Title
  • 01:57Photolithography for Master Mold Fabrication
  • 04:12Soft Lithography for Microchannel Fabrication
  • 06:39Electrotaxis Experiment
  • 08:36Results: Quantification and Analysis of C elegans’ Locomotion during Electrotaxis
  • 09:44Conclusion

Summary

Automatic Translation

Automatic Translation

A semi-automated micro-electro-fluidic method to induce on-demand locomotion in Caenorhabditis elegans is described. This method is based on the neurophysiologic phenomenon of worms responding to mild electric fields (“electrotaxis”) inside microfluidic channels. Microfluidic electrotaxis serves as a rapid, sensitive, low-cost, and scalable technique to screen for factors affecting neuronal health.

Tags

Keywords: MicrofluidicsElectrotaxisCaenorhabditis ElegansLocomotionNeuroprotectiveNeurodegenerative DisordersParkinson’s DiseaseDopaminergic NeuronsScreening SystemElectric FieldSwimming SpeedBody Bending FrequencyReversal TimeAge-related DifferencesPulsed DCAC Electric Fields

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