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Journal / Neuroscience / En simpel cellebaserede immunofluorescens Assay…
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Neuroscience

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JoVE Journal Neuroscience
En simpel cellebaserede immunofluorescens Assay at opdage autoantistof mod N-Methyl-D-aspartat (NMDA) Receptor i blod
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En simpel cellebaserede immunofluorescens Assay at opdage autoantistof mod N-Methyl-D-aspartat (NMDA) Receptor i blod

Article DOI: 10.3791/56676-v • 07:20 min • January 9th, 2018
January 9th, 2018 •
1,2, 1
1Department of Psychiatry, Chang Gung Memorial Hospital-Linkou, 2Department and Graduate Institute of Biomedical Sciences, Chang Gung University

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Vi udtrykte ectopically NR1 underenhed af NMDA-receptor markeret med grønt fluorescerende protein i humane embryonale celler (HEK293) som antigen til at opdage autoantistoffer mod NMDA receptor i blodet hos patienter mistænkt med autoimmun encephalitis. Denne enkle metode kan være egnet til screening i klinisk indstillinger.

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Neurovidenskab sag 131 Anti-NMDA receptor autoantistof NR1 subunit grøn fluorescerende proteiner autoimmune encephalitis celle-baserede immunofluorescens assay differentialdiagnosticering neuropsykiatriske symptomer
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