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Biology

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A Simple High Efficiency Protocol for Pancreatic Islet Isolation from Mice
 

A Simple High Efficiency Protocol for Pancreatic Islet Isolation from Mice

Article doi: 10.3791/57048
August 30th, 2019

Summary August 30th, 2019

This islet isolation protocol described a novel route of collagenase injection to digest the exocrine tissue and a simplified gradient procedure to purify the islets from mice. It involves enzymatic digestion, gradient separation/purification, and islet hand-picking. Successful isolation can yield 250–350 high quality and fully functional islets per mouse.

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