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A High-content In Vitro Pancreatic Islet β-cell Replication Discovery Platform
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A High-content In Vitro Pancreatic Islet β-cell Replication Discovery Platform

A High-content In Vitro Pancreatic Islet β-cell Replication Discovery Platform

DOI:
10.3791/54298-v

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09:35 min

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July 16, 2016

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, , ,
1Department of Medicine, Division of Endocrinology,Stanford University School of Medicine

Chapters

  • 00:05Title
  • 00:57Pancreas Perfusion
  • 01:54Pancreas Dissociation
  • 03:41Islet Purification
  • 05:55Islet Cell Dispersion and Plating and Culture Treatment
  • 08:06Results: Dipyridamole Treatment Induces Beta- But Not Alpha-cell Replication
  • 08:48Conclusion

Summary

Automatic Translation

Automatic Translation

Critical challenges for the diabetes research field are to understand the molecular mechanisms that regulate islet β-cell replication and to develop methods for stimulating β-cell regeneration. Herein a high-content screening method to identify and assess the β-cell replication-promoting activity of small molecules is presented.

Tags

Keywords: In VitroPancreatic Isletβ-cell ReplicationScreening PlatformBeta Cell BiologyRegenerative TherapyDiabetesPancreatic DigestionCollagenase

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