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An Invasive Method for the Activation of the Mouse Dentate Gyrus by High-frequency Stimulation
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JoVE Journal Neuroscience
An Invasive Method for the Activation of the Mouse Dentate Gyrus by High-frequency Stimulation

An Invasive Method for the Activation of the Mouse Dentate Gyrus by High-frequency Stimulation

DOI:
10.3791/57857-v

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12:26 min

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June 02, 2018

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,
1Department of Neurobiology,Beijing Institute of Basic Medical Sciences, 2Key Laboratory of Neuroregeneration, Co-innovation Center of Neuroregeneration,Nantong University

Chapters

  • 00:04Title
  • 01:06Electrode Implantation
  • 05:24High-Frequency Stimulation Delivery
  • 06:56Immunofluorescence Staining and Bromodeoxyuridine Labeling
  • 10:05Results: Distinct activation of neurogenesis in the DG in response to HFS in DG and PP
  • 11:46Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol shows how to set up a reliable HFS method in mice. Neurons throughout the hippocampal dentate gyrus are electrically stimulated by HFS directly and indirectly in vivo. Neuronal activity and molecular signaling are examined by c-fos and Notch1 immunofluorescent staining, respectively; neurogenesis is quantified by bromodeoxyuridine labeling assay.

Tags

Keywords: High-frequency StimulationNeuronal ActivityNeurogenesisHippocampusDeep Brain StimulationMouse Dentate GyrusStereotactic SurgeryElectrode ImplantationBrain Disorders

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