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Functional Characterization of Carboxylesterases in Insecticide Resistant House Flies, Musca Domestica
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Chapters
- 00:04Title
- 00:53Expression and Isolation of Target Proteins with a Baculovirus-mediated Insect Cell Expression System
- 04:18A Cell-based MTT Cytotoxicity Assay
- 06:30Results: MdαE7 Expressing Cells Show Higher Viability when Exposed to Permethrin
- 07:12Conclusion
Here, we present a protocol to produce house fly carboxylesterase proteins in vitro with a baculovirus mediated insect cell expression system and later functionally characterize their roles in metabolizing permethrin, thereby, conferring pyrethroid resistance by conducting cell-based MTT assay and in vitro metabolic studies.
