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Structural Characterization of Mannan Cell Wall Polysaccharides in Plants Using PACE
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Structural Characterization of Mannan Cell Wall Polysaccharides in Plants Using PACE

Structural Characterization of Mannan Cell Wall Polysaccharides in Plants Using PACE

DOI:
10.3791/56424-v

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11:06 min

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October 16, 2017

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, ,
1Joint BioEnergy Institute, 2Environmental and Systems Biology, BioSciences Division,Lawrence Berkeley National Laboratory

Chapters

  • 00:05Title
  • 00:37Aliquoting and Pre-treatment of Alcohol Insoluble Residue (AIR)
  • 02:11Hydrolysis of AIR with Glycosyl Hydrolases
  • 03:20Derivitization of Oligosaccharides
  • 04:29Preparation of PACE Gels
  • 06:33Running a PACE Gel
  • 07:51Visualizing a PACE Gel
  • 09:07Results: Characterization of Plant Cell Wall Glucomannam Structure
  • 10:30Conclusion

Summary

Automatic Translation

Automatic Translation

A protocol for the structural analysis of polysaccharides by gel electrophoresis (PACE), using mannan as an example, is described.

Tags

Keywords: Plant Cell WallPolysaccharidesMannanPACEStructural CharacterizationAIRMannanaseANTS Derivatization

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