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JoVE Journal
Immunology and Infection

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Isolation and Identification of Extravascular Immune Cells of the Heart
 

Isolation and Identification of Extravascular Immune Cells of the Heart

Article doi: 10.3791/58114
August 23rd, 2018

Summary August 23rd, 2018

This protocol presents a simple and efficient method to isolate, identify and quantify immune cells residing in the myocardium of mice during steady state or inflammation. The protocol combines enzymatic and mechanical digestion for the generation of a single cell suspension that can be further analyzed by flow cytometry.

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