Isolation and Culture of Resident Cardiac Macrophages from the Murine Sinoatrial and Atrioventricular Node

Ruibing Xia; Simone Loy; Stefan K&#228;&#228;b; Anna Titova; Christian Schulz; Steffen Massberg; Sebastian Clauss

doi:10.3791/62236

Journal

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Medicine

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Isolation and Culture of Resident Cardiac Macrophages from the Murine Sinoatrial and Atrioventricular Node

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Medicine

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JoVE Journal Medicine

Isolation and Culture of Resident Cardiac Macrophages from the Murine Sinoatrial and Atrioventricular Node

4,252 Views

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08:43 min

•

May 07, 2021

DOI:

10.3791/62236-v

DOI:

10.3791/62236-v

•

08:43 min

May 07, 2021

•

4190 Views

Ruibing Xia^1,2,3, Simone Loy¹, Stefan Kääb^1,3, Anna Titova¹, Christian Schulz^1,2,3, Steffen Massberg^1,2,3, Sebastian Clauss^1,2,3

¹University Hospital Munich, Department of Medicine I,Ludwig-Maximilian-Unversity Munich (LMU)²Insitute of Surgical Research at the Walter Brendel Center of Experimental Medicine, University Hospital Munich,Ludwig-Maximilians-University (LMU)³German Center for Cardiovascular Research (DZHK), Partner Site Munich, Munich Heart Alliance (MHA)

Summary

The protocol presented here provides a step-by-step approach for the isolation of cardiac resident macrophages from the sinoatrial node (SAN) and atrioventricular node (AVN) region of mouse hearts.

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Isolation and Culture of Resident Cardiac Macrophages from the Murine Sinoatrial and Atrioventricular Node

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