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JoVE Journal
Immunology and Infection

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Isolation of Macrophage Subsets and Stromal Cells from Human and Mouse Myocardial Specimens
 

Isolation of Macrophage Subsets and Stromal Cells from Human and Mouse Myocardial Specimens

Article DOI: 10.3791/60015-v 07:25 min December 17th, 2019
December 17th, 2019

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Summary

Presented here is a protocol to isolate various subsets of macrophages and other non-immune cells from human and mouse myocardium by preparing a single cell suspension through enzymatic digestion. Gating schemes for flow cytometry based identification and characterization of isolated macrophages are also presented.

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Isolation Macrophage Subsets Stromal Cells Myocardial Specimens Immune Cells Cardiac Inflammation Cell Diversity Single Cell Suspension Viable Cell Populations Versatile Method Tissue Digestion Apical Wall Lateral Wall Left Ventricle Epicardial Fat Chordae Tendineae Tissue Chunks Euthanizing Mouse Chest Cavity Perfuse Heart
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