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Immunology and Infection
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Isolation of Macrophage Subsets and Stromal Cells from Human and Mouse Myocardial Specimens
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Immunology and Infection
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JoVE Journal Immunology and Infection
Isolation of Macrophage Subsets and Stromal Cells from Human and Mouse Myocardial Specimens

Isolation of Macrophage Subsets and Stromal Cells from Human and Mouse Myocardial Specimens

DOI:
10.3791/60015-v

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07:25 min

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December 17, 2019

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,
1Department of Medicine,Washington University School of Medicine, 2Department of Immunology and Pathology,Washington University School of Medicine, 3Department of Developmental Biology,Washington University School of Medicine

Chapters

  • 00:05Title
  • 01:14Preparation of Human Cardiac Tissue Specimens
  • 01:41Preparation of the Mouse Heart
  • 02:11Preparation of Single Cell Suspension
  • 04:50Antibody Staining
  • 05:33Results: Flow Cytometry Gating Scheme
  • 06:22Conclusion

Summary

Automatic Translation

Automatic Translation

Presented here is a protocol to isolate various subsets of macrophages and other non-immune cells from human and mouse myocardium by preparing a single cell suspension through enzymatic digestion. Gating schemes for flow cytometry based identification and characterization of isolated macrophages are also presented.

Tags

Keywords: Macrophage SubsetsStromal CellsCardiac InflammationCardiac RepairImmune Cell DiversitySingle Cell SuspensionViable Immune CellsNonimmune Cell PopulationsMurine HeartCollagenase IDNase IHyaluronidaseDMEMDigestion

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