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JoVE Journal
Immunology and Infection

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Isolation of Macrophage Subsets and Stromal Cells from Human and Mouse Myocardial Specimens
 

Isolation of Macrophage Subsets and Stromal Cells from Human and Mouse Myocardial Specimens

Article DOI: 10.3791/60015-v
December 17th, 2019

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Summary December 17th, 2019

Presented here is a protocol to isolate various subsets of macrophages and other non-immune cells from human and mouse myocardium by preparing a single cell suspension through enzymatic digestion. Gating schemes for flow cytometry based identification and characterization of isolated macrophages are also presented.

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