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Using High Content Imaging to Quantify Target Engagement in Adherent Cells
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Using High Content Imaging to Quantify Target Engagement in Adherent Cells

Using High Content Imaging to Quantify Target Engagement in Adherent Cells

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07:23 min

November 29, 2018

DOI:

07:23 min
November 29, 2018

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Summary

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Measurements of drug target engagement are central to effective drug development and chemical probe validation. Here, we detail a protocol for measuring drug-target engagement using high content imaging in a microplate-compatible adaption of the cellular thermal shift assay (CETSA).

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