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Probing Nicotinic Acetylcholine Receptor Function in Mouse Brain Slices via Laser Flash Photolysis of Photoactivatable Nicotine
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Probing Nicotinic Acetylcholine Receptor Function in Mouse Brain Slices via Laser Flash Photolysis of Photoactivatable Nicotine

Probing Nicotinic Acetylcholine Receptor Function in Mouse Brain Slices via Laser Flash Photolysis of Photoactivatable Nicotine

DOI:
10.3791/58873-v

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10:48 min

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January 25, 2019

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, , , ,
1Department of Pharmacology,Northwestern University Feinberg School of Medicine, 2Department of Physiology,Northwestern University Feinberg School of Medicine, 3Janelia Research Campus,Howard Hughes Medical Institute

Chapters

  • 00:04Title
  • 00:39Preparation of Photoactivatable Nicotine (PA-Nic)
  • 03:23Imaging Neurons with 2-Photon Laser Scanning Microscopy
  • 05:28Calibration of Uncaging Laser Galvanometers and Pairing of PA-Nic Laser Flash Photolysis with Electrophysiological Recordings
  • 07:47Results: Representative 2-Photon Laser Scanning Microscopy Imaging and PA-Nic Laser Flash Photolysis Responses
  • 09:47Conclusion

Summary

Automatic Translation

Automatic Translation

This article presents a method for studying nicotinic acetylcholine receptors (nAChRs) in mouse brain slices by nicotine uncaging. When coupled with simultaneous patch clamp recording and 2-photon laser scanning microscopy, nicotine uncaging connects nicotinic receptor function with cellular morphology, providing a deeper understanding of cholinergic neurobiology.

Tags

Keywords: Laser Flash PhotolysisPhotoactivatable NicotineNicotinic Acetylcholine ReceptorsMouse Brain SlicesMulti-photon Laser-scanning MicroscopySpatiotemporal ControlCholinergic Synaptic TransmissionVolume TransmissionPatch ClampPressure EjectionBath ApplicationPerfusion System

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