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Single Synapse Indicators of Glutamate Release and Uptake in Acute Brain Slices from Normal and Huntington Mice
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Neuroscience
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JoVE Journal Neuroscience
Single Synapse Indicators of Glutamate Release and Uptake in Acute Brain Slices from Normal and Huntington Mice

Single Synapse Indicators of Glutamate Release and Uptake in Acute Brain Slices from Normal and Huntington Mice

DOI:
10.3791/60113-v

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08:27 min

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March 11, 2020

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,
1Synaptic Dysfunction Group, Neuroscience Research Center,Charité – University Medicine

Chapters

  • 00:04Introduction
  • 00:28Autofluorescence Correction
  • 01:48Bouton Search
  • 03:19Glutamate Release and Clearance Visualization
  • 04:49Off-Line Glutamate Transient Construction and Rapid Glutamate Release and Clearance Assessment
  • 06:01Results: Representative Identification of Dysfunctional Synapses in Q175 Mice with a Hypokinetic Phenotype
  • 07:51Conclusion

Summary

Automatic Translation

Automatic Translation

We present a protocol to evaluate the balance between glutamate release and clearance at single corticostriatal glutamatergic synapses in acute slices from adult mice. This protocol uses the fluorescent sensor iGluu for glutamate detection, a sCMOS camera for signal acquisition and a device for focal laser illumination.

Tags

Keywords: Single SynapseGlutamate ReleaseGlutamate UptakeAcute Brain SlicesHuntington MiceOne-photon MicroscopeAutofluorescence CorrectionDorsal StriatumStimulation PipetteAction Potential Dependent ReleaseIon Channel BlockersMembrane ReceptorsTetrototoxin

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