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Assessment of Ultrastructural Neuroplasticity Parameters After In Utero Transduction of the Developing Mouse Brain and Spinal Cord
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Developmental Biology
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JoVE Journal Developmental Biology
Assessment of Ultrastructural Neuroplasticity Parameters After In Utero Transduction of the Developing Mouse Brain and Spinal Cord

Assessment of Ultrastructural Neuroplasticity Parameters After In Utero Transduction of the Developing Mouse Brain and Spinal Cord

DOI:
10.3791/59084-v

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10:28 min

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February 26, 2019

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, , , , , ,
1Department of Neuroanatomy and Molecular Brain Research,Ruhr University Bochum, 2Central Animal Facility of the Medical Faculty,Ruhr University Bochum

Chapters

  • 00:04Title
  • 01:22In Utero Transduction
  • 03:13Telemacrophotographic Imaging
  • 04:12Transmission Electron Microscopy Tissue Preparation
  • 06:13Ultrastructural Neuroplasticity Parameter Selection
  • 07:44Results: Representative Ultrastructural Imaging of In Utero-Transduced Mouse Embryonic Tissues
  • 09:23Conclusion

Summary

Automatic Translation

Automatic Translation

The combination of transmission electron microscopy and in utero transduction is a powerful approach for studying morphological changes in the fine ultrastructure of the nervous system during development. This combined method allows deep insights into changes in structural details underlying neuroplasticity with respect to their topographical representation.

Tags

Keywords: Ultrastructural NeuroplasticityIn Utero TransductionMouse BrainSpinal CordCongenital Neurodegenerative DiseasesAdeno-associated VirusEmbryonic DevelopmentElectron Microscopy

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