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Genetic Mapping of Thermotolerance Differences Between Species of Saccharomyces Yeast via Genome-Wide Reciprocal Hemizygosity Analysis
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JoVE Journal Genetics
Genetic Mapping of Thermotolerance Differences Between Species of Saccharomyces Yeast via Genome-Wide Reciprocal Hemizygosity Analysis

Genetic Mapping of Thermotolerance Differences Between Species of Saccharomyces Yeast via Genome-Wide Reciprocal Hemizygosity Analysis

DOI:
10.3791/59972-v

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10:08 min

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August 12, 2019

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, ,
1Department of Plant and Microbial Biology,University of California Berkeley, 2Department of Biology,Stanford University, 3Buck Institute for Research on Aging

Chapters

  • 00:04Title
  • 00:22Preparation of Hybrid Yeast Cells for Transformation
  • 01:55Transformation of pJR487 into Hybrid Yeast Cells
  • 08:16Results: Thermotolerance Differences between Species of Saccharomyces
  • 09:44Conclusion

Summary

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Reciprocal hemizygosity via sequencing (RH-seq) is a powerful new method to map the genetic basis of a trait difference between species. Pools of hemizygotes are generated by transposon mutagenesis and their fitness is tracked through competitive growth using high-throughout sequencing. Analysis of the resulting data pinpoints genes underlying the trait.

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Keywords: Genetic MappingThermotoleranceSaccharomyces YeastGenome-wide Reciprocal Hemizygosity AnalysisHigh-throughputHigh ResolutionFreezer StockYPD AgarLiquid YPDOptical DensityBack DilutionTris-EDTA BufferLithium AcetatePolyethylene Glycol

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