Mapping Bacterial Functional Networks and Pathways in <em>Escherichia Coli</em> using Synthetic Genetic Arrays

Alla Gagarinova; Mohan Babu; Jack Greenblatt; Andrew Emili

doi:10.3791/4056

Journal

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Biology

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Mapping Bacterial Functional Networks and Pathways in Escherichia Coli using Synthetic Genetic Arrays

JoVE Journal
Biology

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JoVE Journal Biology

Mapping Bacterial Functional Networks and Pathways in Escherichia Coli using Synthetic Genetic Arrays

Mapping Bacterial Functional Networks and Pathways in Escherichia Coli using Synthetic Genetic Arrays

46,385 Views

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14:06 min

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November 12, 2012

DOI:

10.3791/4056-v

DOI:

10.3791/4056-v

•

14:06 min

November 12, 2012

•

1 Views

Alla Gagarinova*¹, Mohan Babu*^2,3, Jack Greenblatt^1,2, Andrew Emili^1,2

¹Department of Molecular Genetics,University of Toronto, ²Banting and Best Department of Medical Research, Donnelly Centre,University of Toronto, ³Department of Biochemistry, Research and Innovation Centre,University of Regina

Summary

Systematic, large-scale synthetic genetic (gene-gene or epistasis) interaction screens can be used to explore genetic redundancy and pathway cross-talk. Here, we describe a high-throughput quantitative synthetic genetic array screening technology, termed eSGA that we developed for elucidating epistatic relationships and exploring genetic interaction networks in Escherichia coli.