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Chapters
- 00:05Title
- 01:40Constructing Non-essential Query Deletion in an Hfr Cavalli Donor Strain
- 06:42Arraying E. coli F-Recipient Mutants for E. coli Synthetic Genetic Array (eSGA) Screens
- 08:45High-density Strain Mating Procedure to Generate E. coli Double Mutants
- 11:01Results: Representative Analyses of Genetic Interaction (GI) Scores for Determining Pathway-level Functional Relationships
- 13:16Conclusion
Systematic, large-scale synthetic genetic (gene-gene or epistasis) interaction screens can be used to explore genetic redundancy and pathway cross-talk. Here, we describe a high-throughput quantitative synthetic genetic array screening technology, termed eSGA that we developed for elucidating epistatic relationships and exploring genetic interaction networks in Escherichia coli.
Tags
Bacterial Functional NetworksPathwaysEscherichia ColiSynthetic Genetic ArraysPhenotypesPhysical InteractionsFunctional InteractionsGene-gene InteractionsGenetic InteractionsGI ScreensEpistatic DependenciesFunctional RelationshipsLarge-scale GI MapsProkaryotesFunctional AnnotationBacterial GenomesQuantitative Bacterial GI Screening MethodsE. Coli Synthetic Genetic Array (eSGA) Screening ProcedureGenome-scale Screening
