In this work, a robust method for the quantification of mating efficiency in the yeast Saccharomyces cerevisiae is described. This method is particularly useful for the quantification of pre-zygotic barriers in speciation studies.
Mahilkar, A., Nagendra, P., Saini, S. Determination of the Mating Efficiency of Haploids in Saccharomyces cerevisiae. J. Vis. Exp. (190), e64596, doi:10.3791/64596 (2022).