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JoVE Journal
Developmental Biology

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A Layered Mounting Method for Extended Time-Lapse Confocal Microscopy of Whole Zebrafish Embryos
 

A Layered Mounting Method for Extended Time-Lapse Confocal Microscopy of Whole Zebrafish Embryos

Article DOI: 10.3791/60321-v 08:55 min January 14th, 2020
January 14th, 2020

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Summary

This article describes a method to mount fragile zebrafish embryos for extended time-lapse confocal microscopy. This low-cost method is easy to perform using regular glass-bottom microscopy dishes for imaging on any inverted microscope. The mounting is performed in layers of agarose at different concentrations.

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Time-lapse Confocal Microscopy Zebrafish Embryos Mounting Method Unrestricted Growth Fixed Position Inverted Microscope Aquatic Animal Models Breeding Zebrafish Low Melt Agarose Embryo Media Tricaine Toxic Fume Hood E3 Solution Petri Dish Anesthetize Embryos
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