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Identification of Host Pathways Targeted by Bacterial Effector Proteins using Yeast Toxicity and Suppressor Screens
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Genetics
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JoVE Journal Genetics
Identification of Host Pathways Targeted by Bacterial Effector Proteins using Yeast Toxicity and Suppressor Screens

Identification of Host Pathways Targeted by Bacterial Effector Proteins using Yeast Toxicity and Suppressor Screens

DOI:
10.3791/60488-v

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07:40 min

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October 25, 2019

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,
1Department of Microbiology and Immunology,University of Iowa

Chapters

  • 00:04Title
  • 00:46Toxicity Testing of the Protein of Interest
  • 01:53Transformation of Toxic Yeast with the Yeast Genomic Library
  • 05:44Results: Yeast Suppressor Screen
  • 06:48Conclusion

Summary

Automatic Translation

Automatic Translation

Bacterial pathogens secrete proteins into the host that target crucial biological processes. Identifying the host pathways targeted by bacterial effector proteins is key to addressing molecular pathogenesis. Here, a method using a modified yeast suppressor and toxicity screen to elucidate host pathways targeted by toxic bacterial effector proteins is described.

Tags

Keywords: Yeast Toxicity ScreenYeast Suppressor ScreenBacterial Effector ProteinsHost PathwaysChlamydia TrachomatisLegionella PneumophilaCoxiella BurnetiiIntracellular PathogensProtein-protein InteractionsToxicity AssayGrowth AssayPlasmid TransformationDilution SeriesGalactose InductionYeast CultureCentrifugationLithium Acetate

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