Journal
/
Biology
/
Identification of Growth Inhibition Phenotypes Induced by Expression of Bacterial Type III Effectors in Yeast
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
Identification of Growth Inhibition Phenotypes Induced by Expression of Bacterial Type III Effectors in Yeast

Identification of Growth Inhibition Phenotypes Induced by Expression of Bacterial Type III Effectors in Yeast

DOI:
10.3791/1865-v

•

09:34 min

•

March 30, 2010

•

,
1Department of Plant Sciences,Tel Aviv University

Chapters

  • 00:00Title
  • 00:37Introduction
  • 01:10Designing a Yeast Expression System for Type III Effectors
  • 02:13Yeast Transformation
  • 04:21Preparing Yeast Protein Extracts to Verify Effector Experession
  • 06:46Spotting Assay to Detect Effector-induced Growth Inhibition Phenotypes
  • 08:08Spotting Assay Results
  • 09:02Conclusion

Summary

Automatic Translation

Automatic Translation

In this video, we describe a procedure for the expression of bacterial type III effectors in yeast and the identification of effector-induced growth inhibition phenotypes. Such phenotypes can be subsequently exploited to elucidate effector functions and targets.

Tags

Growth Inhibition PhenotypesBacterial Type III EffectorsGram-negative Pathogenic BacteriaType III Secretion SystemEffector ProteinsHost CellsImmune ResponsesPathogen GrowthEffector RepertoireVirulenceInfection StagesGenetic ManipulationBudding Yeast Saccharomyces CerevisiaeFunctional Characterization Of Effector ProteinsCellular ProcessesEukaryotes

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/1865?article_type=v

Bacterial Gene Expression Analysis Using Microarrays
check_url/1865?article_type=v

Expression of Recombinant Proteins in the Methylotrophic Yeast Pichia pastoris
check_url/1865?article_type=v

Bacterial Delivery of RNAi Effectors: Transkingdom RNAi
check_url/1865?article_type=v

Growth Assays to Assess Polyglutamine Toxicity in Yeast
check_url/1865?article_type=v

Temporal Quantification of MAPK Induced Expression in Single Yeast Cells
check_url/1865?article_type=v

Quantification of Orofacial Phenotypes in Xenopus
check_url/1865?article_type=v

Using Microfluidic Devices to Measure Lifespan and Cellular Phenotypes in Single Budding Yeast Cells
check_url/1865?article_type=v

ODELAY: A Large-scale Method for Multi-parameter Quantification of Yeast Growth
check_url/1865?article_type=v

Precise, High-throughput Analysis of Bacterial Growth
check_url/1865?article_type=v

Molecular Analysis of Endothelial-mesenchymal Transition Induced by Transforming Growth Factor-β Signaling

Read Article