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Isolating Potentiated Hsp104 Variants Using Yeast Proteinopathy Models

Article DOI: 10.3791/52089
November 11th, 2014 •
1, 1, 1, 1, 1
1Department of Biochemistry and Biophysics, Perelman School of Medicine at the University of Pennsylvania

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November 11th, 2014

Yeast proteinopathy models are valuable tools to assess the toxicity and aggregation of proteins implicated in disease. Here, we present methods for screening Hsp104 variant libraries for toxicity suppressors. This protocol could be adapted to screen any protein library for toxicity suppressors of any protein that is toxic in yeast.

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