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November 11, 2014
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Yeast proteinopathy models are valuable tools to assess the toxicity and aggregation of proteins implicated in disease. Here, we present methods for screening Hsp104 variant libraries for toxicity suppressors. This protocol could be adapted to screen any protein library for toxicity suppressors of any protein that is toxic in yeast.
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Jackrel, M. E., Tariq, A., Yee, K., Weitzman, R., Shorter, J. Isolating Potentiated Hsp104 Variants Using Yeast Proteinopathy Models. J. Vis. Exp. (93), e52089, doi:10.3791/52089 (2014).
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