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Isolating Potentiated Hsp104 Variants Using Yeast Proteinopathy Models
Isolating Potentiated Hsp104 Variants Using Yeast Proteinopathy Models
JoVE Journal
Biology
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JoVE Journal Biology
Isolating Potentiated Hsp104 Variants Using Yeast Proteinopathy Models

Isolating Potentiated Hsp104 Variants Using Yeast Proteinopathy Models

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08:44 min

November 11, 2014

DOI:

08:44 min
November 11, 2014

8106 Views
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Summary

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Yeast proteinopathy models are valuable tools to assess the toxicity and aggregation of proteins implicated in disease. Here, we present methods for screening Hsp104 variant libraries for toxicity suppressors. This protocol could be adapted to screen any protein library for toxicity suppressors of any protein that is toxic in yeast.

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