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Mating-based Overexpression Library Screening in Yeast
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Genetics
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JoVE Journal Genetics
Mating-based Overexpression Library Screening in Yeast

Mating-based Overexpression Library Screening in Yeast

DOI:
10.3791/57978-v

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11:39 min

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July 06, 2018

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, , , ,
1Department of Biological Sciences,Wright State University

Chapters

  • 00:04Title
  • 00:39Yeast Transformation in 96-well Format
  • 05:48Mating Between Cells Containing Library Genes and Query Yeast
  • 08:20Spotting Assay
  • 09:12Results: Successful Screening for Genes that Modify FUS Toxicity
  • 10:44Conclusion

Summary

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This article presents a mating-based method to facilitate overexpression screening in budding yeast using an arrayed plasmid library.

Tags

Mating-based OverexpressionLibrary ScreeningYeastNeurodegenerative DiseaseGenetic FactorsCellular ProcessesStress ConditionsYeast Peptone DextroseHaploid YeastLithium AcetateSalmon Sperm DNATransformation Buffer

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