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Electromechanical Assessment of Optogenetically Modulated Cardiomyocyte Activity
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Medicine
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JoVE Journal Medicine
Electromechanical Assessment of Optogenetically Modulated Cardiomyocyte Activity

Electromechanical Assessment of Optogenetically Modulated Cardiomyocyte Activity

DOI:
10.3791/60490-v

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12:52 min

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March 05, 2020

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, , , , ,
1Institute for Experimental Cardiovascular Medicine, University Heart Center Freiburg-Bad Krozingen,Medical Center-University of Freiburg, 2Faculty of Medicine,University of Freiburg, 3Faculty of Biology,University of Freiburg

Chapters

  • 00:05Introduction
  • 01:33Cell Isolation
  • 02:57Cardiomyocyte Culture
  • 04:17Patch-Clamp Experiment
  • 05:44Carbon Fiber Preparation
  • 07:40Cardiomyocyte Contraction Force Recording
  • 10:23Results: Representative Patch-Clamp and Carbon Fiber Recordings
  • 11:44Conclusion

Summary

Automatic Translation

Automatic Translation

We present a protocol for evaluating the electromechanical effects of GtACR1 activation in rabbit cardiomyocytes. We provide detailed information on cell isolation, culturing and adenoviral transduction, and on functional experiments with the patch-clamp and carbon-fiber techniques.

Tags

Keywords: OptogeneticsCardiomyocyteElectromechanical AssessmentGtACR1Patch ClampSarcomere TrackingCarbon FiberOptical DefibrillationVentricular CardiomyocytesSmooth MuscleSingle-cell MuscleCell StretchingCell DissociationCell CultureLaminin

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