Parallel Interrogation of β-Arrestin2 Recruitment for Ligand Screening on a GPCR-Wide Scale using PRESTO-Tango Assay

The Presto-Tango platform was devised to execute the parallel and simultaneous interrogation of all non-olfactory GPCRs in the human genome, including that orphan targets to profile ligand-induced beta-arrestin 2 recruitment. The Presto-Tango platform is the only open source resource for profiling the entire GPCR genome in a parallel approach, using a G protein independent beta-arrestin recruitment assay. Demonstrating the processor would be Genevieve Laroche a Research Associate in my lab, and Manel Zighal, a PhD candidate in my lab.

Before beginning the procedure add 20 microliters of a 25 microgram per milliliter poly-L-lysine solution to each well of the appropriate experimental number of 384 optical bottom plates and incubate the plates at room temperature for 30 minutes to two hours. At the end of the incubation, flick the plates over the sink to remove the excess poly-L-lysine and add 40 microliters of antibiotic antimycotic solution to each well. Then incubate the plates needed for cell seeding at 37 degrees Celsius and place the rest of the plates at four degrees Celsius for long term storage.