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Standardized Methods for Measuring Induction of the Heat Shock Response in Caenorhabditis elegans
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Standardized Methods for Measuring Induction of the Heat Shock Response in Caenorhabditis elegans

Article DOI: 10.3791/61030-v • 06:01 min • July 3rd, 2020
July 3rd, 2020 •
1, 1, 1, 1
1Department of Biomedical and Chemical Engineering and Sciences, Florida Institute of Technology

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Here, standardized protocols are presented to assess induction of the heat shock response (HSR) in Caenorhabditis elegans using RT-qPCR at the molecular level, fluorescent reporters at the cellular level, and thermorecovery at the organismal level.

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Standardized Methods Measuring Induction Heat Shock Response Caenorhabditis Elegans Cellular Stress Response Molecular Level Cellular Level Organismal Level Protocols Best Practices Reproducibility Thermo Tolerance Thermal Recovery Heat Shock Response Dependent Attenuated Egg Laying Developmental Timing Synchronized Worm Population Gravid Adult Worms Fresh Plate Larvae Removal Developmental Stage Temperature Variation
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