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Biology

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Detecting Protein Subcellular Localization by Green Fluorescence Protein Tagging and 4',6-Diamidino-2-phenylindole Staining in Caenorhabditis elegans
 

Detecting Protein Subcellular Localization by Green Fluorescence Protein Tagging and 4',6-Diamidino-2-phenylindole Staining in Caenorhabditis elegans

Article DOI: 10.3791/57914 09:36 min July 30th, 2018
July 30th, 2018

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Summary

This protocol demonstrates how to track a protein nuclear translocation under heat stress by using a green fluorescence protein (GFP) fusion protein as a marker and 4',6-diamidino-2-phenylindole (DAPI) staining. The DAPI staining protocol is fast and preserves the GFP and protein subcellular localization signals.

Transcript

Tags

Protein Subcellular Localization Green Fluorescence Protein Tagging 4',6-Diamidino-2-phenylindole Staining Caenorhabditis Elegans Cell Biology Nuclear Staining GFP Sub-cellular Localization Changes Extrachromosomal Array Translational Construct Caenorhabditis Genetic Center Radiation Exposure Marker Protein Outcrossing NGM Worm Plates OP50 Clone Liquid LB Broth Bacteria Lawn
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