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JoVE Journal
Immunology and Infection

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Simplified Reverse Genetics Method to Recover Recombinant Rotaviruses Expressing Reporter Proteins
 

Simplified Reverse Genetics Method to Recover Recombinant Rotaviruses Expressing Reporter Proteins

Article doi: 10.3791/61039
April 17th, 2020 Usage Statistics

Summary April 17th, 2020

Generation of recombinant rotaviruses from plasmid DNA provides an essential tool for the study of rotavirus replication and pathogenesis, and the development of rotavirus expression vectors and vaccines. Herein, we describe a simplified reverse genetics approach for generating recombinant rotaviruses, including strains expressing fluorescent reporter proteins.

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