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Methods to Study Changes in Inherent Protein Aggregation with Age in Caenorhabditis elegans
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Methods to Study Changes in Inherent Protein Aggregation with Age in Caenorhabditis elegans

Methods to Study Changes in Inherent Protein Aggregation with Age in Caenorhabditis elegans

DOI:
10.3791/56464-v

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11:57 min

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November 26, 2017

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, , , , ,
1Protein Aggregation and Aging,German Center for Neurodegenerative Diseases (DZNE), 2Graduate School of Cellular and Molecular Neuroscience,German Center for Neurodegenerative Diseases (DZNE)

Chapters

  • 00:05Title
  • 00:53Liquid Culture for Treatment with RNAi Starting at L1
  • 02:40Collection of Young and Aged Worms Subjected to RNAi in Liquid Culture
  • 06:14Disruption of Animals
  • 06:58Isolation of SDS-insoluble Proteins for Mass Spectrometry
  • 10:03Results: Reduced IIS Prevents Age-dependent Protein Aggregation
  • 11:26Conclusion

Summary

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The goal of the method presented here is to explore protein aggregation during normal aging in the model organism C. elegans. The protocol represents a powerful tool to study the highly insoluble large aggregates that form with age and to determine how changes in proteostasis impact protein aggregation.

Tags

Keywords: Caenorhabditis ElegansProtein AggregationAgingProteostasisInherent Protein AggregationProtein InsolubilityModel OrganismGene KnockdownRNAiWorm CultureWorm CollectionM9 BufferCentrifugationProtein Solubility

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