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Developmental Biology

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A Drosophila Model to Study Wound-induced Polyploidization

Article DOI: 10.3791/61252-v • 07:27 min • June 9th, 2020
June 9th, 2020 •
*1, *1, 2, 1, 1
1Biology Department, Boston College, 2Graduate School of Biomedical Sciences and Engineering and Kathryn W. Davis Center for Regenerative Biology and Medicine, MDI Biological Laboratory, University of Maine
* These authors contributed equally

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Summary
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Wound-induced polyploidization is a conserved tissue repair strategy where cells grow in size instead of dividing to compensate for cell loss. Here is a detailed protocol on how to use the fruit fly as a model to measure ploidy and its genetic regulation in epithelial wound repair.

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Drosophila Model Wound-induced Polyploidization Tissue Repair Process Adult Fruit Fly Puncture Wound Fly Epithelium Epithelial Cell Size Ploidy And Organization Vials Female Fruit Flies Strain Of Interest Aging The Flies Fresh Food Vials Male Flies Incubation Stainless Steel Pin Pin Holders Anesthetize The Flies Carbon Dioxide Fly Pad Stereo Microscope Paintbrush Ventral Abdomens Target A4 Region Ventral Midline Stir Nights Experimental Time Point Post-injury Wound And Scar
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