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Mitigation of Blood Borne Cell Attachment to Metal Implants through CD47-Derived Peptide Immobilization
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Bioengineering
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JoVE Journal Bioengineering
Mitigation of Blood Borne Cell Attachment to Metal Implants through CD47-Derived Peptide Immobilization

Mitigation of Blood Borne Cell Attachment to Metal Implants through CD47-Derived Peptide Immobilization

DOI:
10.3791/61545-v

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08:13 min

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December 03, 2020

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, , , , ,
1The Children’s Hospital of Philadelphia, 2Department of Pediatrics, Perelman School of Medicine,University of Pennsylvania

Chapters

  • 00:04Introduction
  • 00:50Coating Bare Metal Surfaces with PEI-PDT
  • 02:37Attaching Human PepCD47 to PEI-PDT Modified Surfaces
  • 03:21Chandler Loop for Analyzing Cellular Attachment to Metal Surfaces
  • 04:54Analyzing Cellular Attachment to Metal Surfaces using CFDA Dye
  • 05:48Results: Anti-inflammatory Properties of PepCD47 Coated Metal Surfaces Exposed to Whole Blood and Isolated Monocytes
  • 07:16Conclusion

Summary

Automatic Translation

Automatic Translation

Presented here is a protocol for appending peptide CD47 (pepCD47) to metal stents using polybisphosphonate chemistry. Functionalization of metal stents using pepCD47 prevents the attachment and activation of inflammatory cells thus improving their biocompatibility.

Tags

Keywords: Metal ImplantsCD47Peptide ImmobilizationBiocompatibilityStainless SteelPABTTCEPPEI-PDTPeptide ConjugationCD47 Peptide

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