The Use of the <em>Ex Vivo</em> Chandler Loop Apparatus to Assess the Biocompatibility of Modified Polymeric Blood Conduits

Joshua B. Slee; Ivan S. Alferiev; Robert J. Levy; Stanley J. Stachelek

doi:10.3791/51871

Journal

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Bioengineering

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The Use of the Ex Vivo Chandler Loop Apparatus to Assess the Biocompatibility of Modified Polymeric Blood Conduits

The Use of the <em>Ex Vivo</em> Chandler Loop Apparatus to Assess the Biocompatibility of Modified Polymeric Blood Conduits

JoVE Journal
Bioengineering

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JoVE Journal Bioengineering

The Use of the Ex Vivo Chandler Loop Apparatus to Assess the Biocompatibility of Modified Polymeric Blood Conduits

The Use of the Ex Vivo Chandler Loop Apparatus to Assess the Biocompatibility of Modified Polymeric Blood Conduits

11,616 Views

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10:15 min

•

August 20, 2014

DOI:

10.3791/51871-v

DOI:

10.3791/51871-v

•

10:15 min

August 20, 2014

•

11573 Views

Joshua B. Slee^1,2, Ivan S. Alferiev^1,2, Robert J. Levy^1,2, Stanley J. Stachelek^1,2

¹Division of Cardiology, Department of Pediatrics,The Children’s Hospital of Philadelphia ²University of Pennsylvania Perelman School of Medicine

Summary

Blood exposure to polymeric blood conduits initiates the foreign body reaction that has been implicated in clinical complications. Here, the Chandler Loop Apparatus, an experimental tool mimicking blood perfusion through these conduits, is described. Appendage of recombinant CD47 results in decreased evidence of the foreign body reaction on these conduits.