Biology
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Generation of Enterobacter sp. YSU Auxotrophs Using Transposon Mutagenesis
Chapters
Summary October 31st, 2014
Enterobacter sp. YSU grows in glucose minimal salts medium. Auxotrophs are generated by transforming it with a transposome which randomly inserts itself into the host genome. Mutants are found by replica plating from complex medium to minimal medium. Interrupted genes are identified by gene rescue and sequencing.
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