A subscription to JoVE is required to view this content.
You will only be able to see the first 2 minutes.
Summary October 31st, 2014
Enterobacter sp. YSU grows in glucose minimal salts medium. Auxotrophs are generated by transforming it with a transposome which randomly inserts itself into the host genome. Mutants are found by replica plating from complex medium to minimal medium. Interrupted genes are identified by gene rescue and sequencing.