Single-Cell Multiplexed Fluorescence Imaging to Visualize Viral Nucleic Acids and Proteins and Monitor HIV, HTLV, HBV, HCV, Zika Virus, and Influenza Infection

Raven Shah; Shuiyun Lan; Maritza  N. Puray-Chavez; Dandan Liu; Philip  R. Tedbury; Stefan G. Sarafianos

doi:10.3791/61843

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Single-Cell Multiplexed Fluorescence Imaging to Visualize Viral Nucleic Acids and Proteins and Monitor HIV, HTLV, HBV, HCV, Zika Virus, and Influenza Infection

JoVE Journal
Biology

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JoVE Journal Biology

Single-Cell Multiplexed Fluorescence Imaging to Visualize Viral Nucleic Acids and Proteins and Monitor HIV, HTLV, HBV, HCV, Zika Virus, and Influenza Infection

2,724 Views

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07:24 min

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October 29, 2020

DOI:

10.3791/61843-v

DOI:

10.3791/61843-v

•

07:24 min

October 29, 2020

•

2705 Views

Raven Shah*¹, Shuiyun Lan*¹, Maritza N. Puray-Chavez², Dandan Liu², Philip R. Tedbury¹, Stefan G. Sarafianos¹

¹Laboratory of Biochemical Pharmacology, Department of Pediatrics,Emory University School of Medicine ²Department of Molecular Microbiology & Immunology,University of Missouri School of Medicine

Summary

Presented here is a protocol for a fluorescence imaging approach, multiplex immunofluorescent cell-based detection of DNA, RNA, and protein (MICDDRP), a method capable of simultaneous fluorescence single-cell visualization of viral protein and nucleic acids of different type and strandedness. This approach can be applied to a diverse range of systems.