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In vivo Imaging of Fully Active Brain Tissue in Awake Zebrafish Larvae and Juveniles by Skull and Skin Removal
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Chapters
- 00:04Introduction
- 00:57Larva Preparation
- 01:39Larva Embedding
- 02:22Brain Exposure
- 03:21Results: Representative In Vivo Fully Active Awake Zebrafish Larva and Juvenile Brain Tissue Imaging
- 04:26Conclusion
Here we present a method to image the zebrafish embryonic brain in vivo upto larval and juvenile stages. This microinvasive procedure, adapted from electrophysiological approaches, provides access to cellular and subcellular details of mature neuron and can be combined with optogenetics and neuropharmacological studies for characterizing brain function and drug intervention.
