Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biochemistry / Making Precise and Accurate Single-Molecule FRET…
JoVE Journal
Biochemistry

This content is Open Access.

JoVE Journal Biochemistry
Making Precise and Accurate Single-Molecule FRET Measurements using the Open-Source smfBox
Calibration-free In Vitro Quantification of Protein Homo-oligomerization Using Commercial Instrumentation and Free, Open Source Brightness Analysis Software
Journal (Biochemistry)
Calibration-free In Vitro Quantification of Protein Homo-oligomerization Using Commercial Instrumentation and Free, Open Source Brightness Analysis Software
Rab10 Phosphorylation Detection by LRRK2 Activity Using SDS-PAGE with a Phosphate-binding Tag
Journal (Biochemistry)
Rab10 Phosphorylation Detection by LRRK2 Activity Using SDS-PAGE with a Phosphate-binding Tag
 

Making Precise and Accurate Single-Molecule FRET Measurements using the Open-Source smfBox

Article DOI: 10.3791/62378-v • 07:12 min • July 5th, 2021
July 5th, 2021 •
*1, *1, 1, 1
1Sheffield Institute for Nucleic Acids, Department of Chemistry, University of Sheffield
* These authors contributed equally

Chapters

Summary
Automatic Translation

This article provides step-by-step instructions for making fully-corrected accurate FRET measurements on individual, freely diffusing biomolecules using the open-source, inexpensive smfBox, from switch on, through alignment and focusing, to data collection and analysis.

Tags

Single-molecule FRET Open-source SmfBox Biomolecules SmFRET Conformations Protein Folding Protein DNA Interactions DNA Allostery Laser Control Center SmOTTER Acquisition Software Cy3B Dye Laser Duty Cycles Panel ALEX Period Z Focus Ta
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter