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Journal / Bioengineering / Microinjection of Corn Planthopper, Peregrinus…
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Bioengineering

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JoVE Journal Bioengineering
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Microinjection of Corn Planthopper, Peregrinus maidis, Embryos for CRISPR/Cas9 Genome Editing

Article DOI: 10.3791/62417-v • 07:27 min • March 26th, 2021
March 26th, 2021 •
*1, *1, 1, 1, 1, 1, 1
1Department of Entomology and Plant Pathology, North Carolina State University
* These authors contributed equally

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Summary
Automatic Translation

Herein are protocols for collecting and microinjecting precellular corn planthopper embryos for the purpose of modifying their genome via CRISPR/Cas9-based genome editing or for the addition of marked transposable elements through germline transformation.

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Microinjection Corn Planthopper Peregrinus Maidis CRISPR/Cas9 Genome Editing Hemipterans Germline Transformation Insect Embryos Desiccation Sensitivity Transgenic Planthoppers Virus Transmission Control Gene Function Study Western Flower Thrips CRISPR Technique Adaptation Thrips Embryos Chamber Construction Adult Peregrinus Maidis Insects Female Identification
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