Journal
/
Genetics
/
Embryo Injections for CRISPR-Mediated Mutagenesis in the Ant Harpegnathos saltator
JoVE Journal
Genetics
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Genetics
Embryo Injections for CRISPR-Mediated Mutagenesis in the Ant Harpegnathos saltator

Embryo Injections for CRISPR-Mediated Mutagenesis in the Ant Harpegnathos saltator

DOI:
10.3791/61930-v

•

08:30 min

•

February 09, 2021

•

, , , , ,
1Department of Biology,University of Florida, 2Department of Biochemistry and Molecular Pharmacology,New York University School of Medicine, 3Department of Radiation Oncology,Columbia University Medical Center, 4Center for Smell and Taste,University of Florida

Chapters

  • 00:05Introduction
  • 00:40Regular Maintenance of Harpegnathos saltator Colonies
  • 02:05Preparation of Quartz Glass Microinjection Needles and Microinjector
  • 04:10Injection of Embryos
  • 06:32Results: Mutagenesis Induction in H. saltator Ants
  • 07:37Conclusion

Summary

Automatic Translation

Automatic Translation

Many characteristics of insect eusociality rely on within-colony communication and division of labor. Genetic manipulation of key regulatory genes in ant embryos via microinjection and CRISPR-mediated mutagenesis provides insights into the nature of altruistic behavior in eusocial insects.

Tags

Keywords: CRISPRMutagenesisHarpegnathos SaltatorAntGenetic ModificationEmbryo InjectionMicroinjectionPlaster Nest BoxEusocial InsectsBehaviorCommunicationPhenotype PlasticityGraduate StudentAnt RearingColony MaintenanceWaste DisposalGlass Microinjection NeedlesCasein ProteinsSmall Guide RNAs

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/61930?article_type=v

Delivery of Nucleic Acids through Embryo Microinjection in the Worldwide Agricultural Pest Insect, Ceratitis capitata
check_url/61930?article_type=v

Optogenetic Random Mutagenesis Using Histone-miniSOG in C. elegans
check_url/61930?article_type=v

Detection of Copy Number Alterations Using Single Cell Sequencing
check_url/61930?article_type=v

Targeted in Situ Mutagenesis of Histone Genes in Budding Yeast
check_url/61930?article_type=v

Embryo Microinjection and Transplantation Technique for Nasonia vitripennis Genome Manipulation
check_url/61930?article_type=v

CRISPR-Mediated Reorganization of Chromatin Loop Structure
check_url/61930?article_type=v

Gene-targeted Random Mutagenesis to Select Heterochromatin-destabilizing Proteasome Mutants in Fission Yeast
check_url/61930?article_type=v

A Novel Saturation Mutagenesis Approach: Single Step Characterization of Regulatory Protein Binding Sites in RNA Using Phosphorothioates
check_url/61930?article_type=v

Induction and Evaluation of Inbreeding Crosses Using the Ant, Vollenhovia Emeryi
check_url/61930?article_type=v

CRISPR-mediated Genome Editing of the Human Fungal Pathogen Candida albicans

Read Article