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Using Optogenetics to Reverse Neuroplasticity and Inhibit Cocaine Seeking in Rats
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Using Optogenetics to Reverse Neuroplasticity and Inhibit Cocaine Seeking in Rats

Article DOI: 10.3791/63185-v • 09:43 min • October 5th, 2021
October 5th, 2021 •
1,2, 1, 1
1Department of Psychiatry, University of Pittsburgh, 2Department of Psychiatry, Rutgers University

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Summary
Automatic Translation

The methods described here outline a procedure used to optogenetically reverse cocaine-induced plasticity in a behaviorally-relevant circuit in rats. Sustained low-frequency optical stimulation of thalamo-amygdala synapses induces long-term depression (LTD). In vivo optogenetically-induced LTD in cocaine-experienced rats resulted in the subsequent attenuation of cue-motivated drug seeking.

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Optogenetics Neuroplasticity Cocaine Seeking Rats Synaptic Activity Circuit Manipulation Relapse Cue Motivated Behavior Surgical Preparation Injection Cannula AAV Solution Anesthesia Analgesic Carprofen Lactated Ringer's Solution Intravenous Catheter Implantation Stereotaxic Frame Viral Injections
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