Generation of Hook Ischemia-Reperfusion Model using a Three-Day Developing Chick Embryo

Neha Kumari; Santosh Kumar Yadav; Ravi Prakash; Abu Junaid Siddiqui; Mohsin Ali Khan; Syed Shadab Raza

doi:10.3791/63288

Generation of Hook Ischemia-Reperfusion Model using a Three-Day Developing Chick Embryo

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Generation of Hook Ischemia-Reperfusion Model using a Three-Day Developing Chick Embryo

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14:05 min

February 19, 2022

DOI: 10.3791/63288-v

Neha Kumari¹, Santosh Kumar Yadav*¹, Ravi Prakash*¹, Abu Junaid Siddiqui¹, Mohsin Ali Khan², Syed Shadab Raza^1,3

¹Laboratory for Stem Cell and Restorative Neurology, Department of Biotechnology, Era’s Lucknow Medical College Hospital,Era University, ²Era University, ³Department of Stem Cell Biology and Regenerative Medicine,Era University

Overview

This study introduces a cost-effective and reproducible model of ischemia-reperfusion (I/R) injury using a 3-day chick embryo to explore the underlying mechanisms and potential interventions for ischemia-reperfusion disorders.

Key Study Components

Research Area

Ischemia-reperfusion injury
Chick embryo models
Pathophysiological mechanisms in stroke

Background

Understanding I/R disorders is crucial for developing effective treatments.
Existing models can be expensive and time-consuming.
A simple and efficient model can enhance research capabilities.

Methods Used

Utilization of a modified spinal needle for I/R creation
Chick embryo as a biological system
In vivo techniques for monitoring blood flow and I/R effects

Main Results

Demonstrated significant decrease in blood flow during ischemia and restoration during reperfusion.
Validated the model's effectiveness for studying ischemic-reperfusion injury.
Potential for testing therapeutic interventions on arterial tissues.

Conclusions

This model effectively replicates I/R injury and offers insights into therapeutic options.
Highlights the importance of chick embryo models in cardiovascular research.

Frequently Asked Questions

What is the main objective of the study?

To develop a simple model for studying ischemia-reperfusion injury in a chick embryo.

How does the chick embryo model enhance research?

It is cost-effective, easy to reproduce, and allows for real-time monitoring of I/R effects.

What methods are used in the study?

The use of a customized spinal needle to induce I/R and Doppler imaging for blood flow measurement.

What are the implications of the findings?

They provide a foundation for future research on ischemia-reperfusion disorders and potential treatments.

How is the model validated?

Through quantifiable changes in blood flow and biochemical assays of tissue response.

What treatments can be tested using this model?

Interventions targeting oxidative stress and cellular responses to I/R injury.

Are there any limitations to this model?

The results may not fully replicate human conditions, but they provide valuable insights.

This paper describes ischemia-reperfusion (I/R) modeling in a 3-day chick embryo using a spinal needle customized hook to better understand I/R development and treatment. This model is simple, quick, and inexpensive.

Hi, I am Dr.Syed Shadab Raza, the principal investigator for the Laboratory for Stem Cells and Restorative Neurology, located at the Department of Biotechnology at Era University Campus, Lucknow, India. In my laboratory, we strive to understand the pathophysiological mechanism underlying ischemia-reperfusion disorders and the interventions that can reverse those processes. In this context, we frequently employ the in vitro and the in vivo model of ischemic stroke.

Recently, we have created a ischemic-reperfusion injury model in a three day developing chick embryo. So now my students are going to show you how to replicate the ischemia-reperfusion injury in a three day developing chick embryo. Now we are going to show you how to create ischemia-reperfusion in a three days chick embryo.

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