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Journal / Biochemistry / Quantification of Subcellular Glycogen…
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Biochemistry

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JoVE Journal Biochemistry
Quantification of Subcellular Glycogen Distribution in Skeletal Muscle Fibers using Transmission Electron Microscopy
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Quantification of Subcellular Glycogen Distribution in Skeletal Muscle Fibers using Transmission Electron Microscopy

Article DOI: 10.3791/63347-v • 08:32 min • February 7th, 2022
February 7th, 2022 •
1, 2, 3, 3, 2
1Research center for applied health science, University College South Denmark, 2Department of Sports Science and Clinical Biomechanics, University of Southern Denmark, 3Department of Biomedical Sciences, Core Facility for Integrated Microscopy, University of Copenhagen

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Summary
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A modified post-fixation procedure increases the contrast of glycogen particles in tissue. This paper provides a step-by-step protocol describing how to handle the tissue, conduct the imaging, and use stereological methods to obtain unbiased and quantitative data on fiber type-specific subcellular glycogen distribution in skeletal muscle.

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Quantification Subcellular Glycogen Distribution Skeletal Muscle Fibers Transmission Electron Microscopy Measure Glycogen Content Single Cells Subcellular Organizations Physiological Conditions Diseases Technique High Resolution Transmission Electron Microscope Localized Subcellular Structures Glycogen Staining Antibody Free Contrast Potassium Ferrocyanide Fixative Solution Glutaraldehyde Sodium Cacodylate Buffer Muscle Biopsy Whole Muscle Specimen Primary Fixation Solution Wash Specimen
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